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Ham's F-10 Nutrient Mix Liquid media Concentration (mg/L)

Ham's F-10 Nutrient Mixture (F-10) was designed for serum-free growth of Chinese Hamster Ovary (CHO) cells. F-10 has since been used for serum-free growth of CHO cultures as well as serum-supplemented growth of other mammalian cells, including COS-7, primary rat astrocytes, and rat prostate epithelial cells.

Amino Acids

Glycine	7.5	7.5
L-Alanine	8.92	8.92
L-Arginine hydrochloride	211	211
L-Asparagine-H2O	15	15
L-Aspartic acid	13.2	13.2
L-Cysteine	25	25
L-Glutamic Acid	14.7	14.7
L-Glutamine	146	146
L-Histidine hydrochloride-H2O	23	23
L-Isoleucine	2.6	2.6
L-Leucine	13.1	13.1
L-Lysine hydrochloride	29.3	29.3
L-Methionine	4.49	4.49
L-Phenylalanine	5	5
L-Proline	11.5	11.5
L-Serine	10.5	10.5
L-Threonine	3.6	3.6
L-Tryptophan	0.6	0.6
L-Tyrosine disodium salt dihydrate	2.62	2.62
L-Valine	3.5	3.5

Vitamins

D-Biotin	0.02	0.02
Choline chloride	0.71	0.71
D-Calcium pantothenate	0.7	0.7
Folic Acid	1.32	1.32
Niacinamide	0.61	0.61
Pyridoxine hydrochloride	0.2	0.2
Riboflavin	0.39	0.39
Thiamine hydrochloride	1.01	1.01
Vitamin B12	1.36	1.36
Thymidine	0.73	0.73
Lipoic Acid	0.2	0.2
Hypoxanthine Na	5.4	5.4
i-Inositol	0.5	0.5

Inorganic Salts

Calcium Chloride (CaCl2) (anhyd.)	33.29	33.29
Cupric sulfate (CuSO4-5H2O)	0.0025	0.0025
Ferric sulfate (FeSO4-7H2O)	0.834	0.834
Magnesium Sulfate (MgSO4) (anhyd.)	74.64	74.64
Potassium Chloride (KCl)	285	285
Potassium Phosphate monobasic (KH2PO4)	83	83
Sodium Chloride (NaCl)	7400	7400
Sodium Phosphate dibasic (Na2HPO4) anhydrous	153.7	153.7
Zinc sulfate (ZnSO4-7H2O)	0.03	0.03