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McCoy's 5A Medium Liquid media Concentration (mg/L)

McCoy's 5A (modified) Medium is a general purpose medium that supports the propagation of many types of primary cells, established cell lines, and explants from biopsy tissues. This medium will support the growth of primary mammalian cells derived from normal bone marrow, skin, spleen, kidney, lung, rat embryos, and other tissues.

Amino Acids

Glycine	7.51	7.51	7.51
L-Alanine	13.9	13.9	13.9
L-Arginine hydrochloride	42.1	42.1	42.1
L-Asparagine-H2O	45	45	45
L-Aspartic acid	19.97	19.97	19.97
L-Cysteine hydrochloride-H2O	35	35	35
L-Glutamic Acid	22.1	22.1	22.1
L-Glutamine	219.2	219.2	219.2
L-Histidine hydrochloride-H2O	20.96	20.96	20.96
L-Hydroxy proline	19.7	19.7	19.7
L-Isoleucine	39.36	39.36	39.36
L-Leucine	39.36	39.36	39.36
L-Lysine hydrochloride	36.5	36.5	36.5
L-Methionine	14.9	14.9	14.9
L-Phenylalanine	16.5	16.5	16.5
L-Proline	17.28	17.28	17.28
L-Serine	26.29	26.29	26.29
L-Threonine	17.87	17.87	17.87
L-Tryptophan	3.06	3.06	3.06
L-Tyrosine -2Na-2H20	26.1	26.1	26.1
L-Valine	17.6	17.6	17.6

Vitamins

Ascorbic Acid	0.5	0.5	0.5
Biotin	0.2	0.2	0.2
Choline chloride	5	5	5
D-Calcium pantothenate	0.2	0.2	0.2
Folic Acid	10	10	10
Niacinamide	0.5	0.5	0.5
Nicotinic acid (Niacin)	0.5	0.5	0.5
Para-Aminobenzoic Acid	1	1	1
Pyridoxal hydrochloride	0.5	0.5	0.5
Pyridoxine hydrochloride	0.5	0.5	0.5
Riboflavin	0.2	0.2	0.2
Thiamine hydrochloride	0.2	0.2	0.2
Vitamin B12	2	2	2
i-Inositol	36	36	36

Inorganic Salts

Calcium Chloride (CaCl2) (anhyd.)	100	100	100
Magnesium Sulfate (anhyd)	97.7	97.7	97.7
Potassium Chloride (KCl)	400	400	400
Sodium Chloride (NaCl)	6460	6460	6460
Sodium Phosphate monobasic (NaH2PO4-H2O)	580	580	580