Ham's F-12 Nutrient Mixture F12 Liquid media Concentration (mg/L)
Ham's F-12 Nutrient Mixture (F-12) was designed for serum-free, single-cell plating of Chinese Hamster Ovary (CHO) cells. F-12 has since been used for serum-free growth of CHO cultures as well as serum-supplemented growth of other mammalian cells, including chondrocytes and rat prostate epithelial cells.
Amino Acids
|
Glycine |
7.5 |
|
L-Alanine |
8.91 |
|
L-Arginine hydrochloride |
211 |
|
L-Asparagine-H2O |
15.01 |
|
L-Aspartic acid |
13.3 |
|
L-Cysteine hydrochloride-H2O |
35.12 |
|
L-Glutamic Acid |
14.7 |
|
L-Glutamine |
146 |
|
L-Histidine hydrochloride-H2O |
21 |
|
L-Isoleucine |
3.96 |
|
L-Leucine |
13.1 |
|
L-Lysine hydrochloride |
36.55 |
|
L-Methionine |
4.5 |
|
L-Phenylalanine |
5 |
|
L-Proline |
34.5 |
|
L-Serine |
10.5 |
|
L-Threonine |
11.9 |
|
L-Tyrosine disodium salt dihydrate |
7.8 |
|
L-Valine |
11.7 |
|
D-Biotin |
0.0073 |
|
Choline chloride |
14 |
|
D-Calcium pantothenate |
0.5 |
|
Folic Acid |
1.3 |
|
Niacinamide |
0.04 |
|
Pyridoxine hydrochloride |
0.06 |
|
Riboflavin |
0.038 |
|
Thiamine hydrochloride |
0.34 |
|
Vitamin B12 |
1.4 |
|
i-Inositol |
18 |
|
Calcium Chloride (CaCl2) (anhyd.) |
33.22 |
|
Cupric sulfate (CuSO4-5H2O) |
0.0025 |
|
Ferric sulfate (FeSO4-7H2O) |
0.834 |
|
Magnesium Chloride (anhydrous) |
57.22 |
|
Potassium Chloride (KCl) |
223.6 |
|
Sodium Bicarbonate (NaHCO3) |
1176 |
|
Sodium Chloride (NaCl) |
7599 |
|
Sodium Phosphate dibasic (Na2HPO4) anhydrous |
142 |
|
Zinc sulfate (ZnSO4-7H2O) |
0.863 |
D-Glucose (Dextrose)
1802
Hypoxanthine Na
5.4
Linoleic Acid
0.084
Lipoic Acid
0.21
Phenol Red
1.2
Putrescine 2HCl
0.161
Sodium Pyruvate
110
Thymidine
0.7
