Upsilon biosciences

RPMI 1640 Medium

RPMI 1640 Medium was originally developed to culture human leukemic cells in suspension and as a monolayer. Roswell Park Memorial Institute (RPMI) 1640 Medium has since been found suitable for a variety of mammalian cells, including HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes, and carcinomas. We offer a variety of RPMI 1640 Medium modifications for a range of cell culture applications.

Amino Acids

Glycine	10	10	10	10
L-Arginine	200	200	200	200
L-Asparagine	50	50	50	50
L-Asparagine	20	20	20	20
L-Cystine 2HCl	65	65	65	65
L-Glutamic Acid	20	20	20	20
L-Histidine	15	15	15	15
L-Hydroxyproline	20	20	20	20
L-Isoleucine	50	50	50	50
L-Leucine	50	50	50	50
L-Lysine hydrochloride	40	40	40	40
L-Methionine	15	15	15	15
L-Phenylalanine	15	15	15	15
L-Proline	20	20	20	20
L-Serine	30	30	30	30
L-Threonine	20	20	20	20
L-Tryptophan	5	5	5	5
L-Tyrosine disodium salt	29	29	29	29
L-Valine	20	20	20	20

Vitamins

Biotin	0.2	0.2	0.2	0.2
Choline chloride	3	3	3	3
D-Calcium pantothenate	0.25	0.25	0.25	0.25
Folic Acid	1	1	1	1
Niacinamide	1	1	1	1
Para-Aminobenzoic Acid	1	1	1	1
Pyridoxine hydrochloride	1	1	1	1
Riboflavin	0.2	0.2	0.2	0.2
Thiamine hydrochloride	1	1	1	1
Vitamin B12	0.005	0.005	0.005	0.005
i-Inositol	35	35	35	35

Inorganic Salts

Calcium nitrate (Ca(NO3)2 4H2O)	100	100	100	100
Magnesium Sulfate (MgSO4)(anhyd.)	48.84	48.84	48.84	48.84
Potassium Chloride (KCl)	400	400	400	400
Sodium Bicarbonate (NaHCO3)	2000	2000	2000	2000
Sodium Chloride (NaCl)	6000	6000	6000	6000
Sodium Phosphate dibasic (Na2HPO4-7H2O)	1512	1512	1512	1512