Dulbecco's Modified Eagle's Medium - High Glucose
DMEM (Dulbecco's Modified Eagle Medium) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12.
Amino Acids
|
Glycine
|
30
|
30 |
30 |
30 |
30 |
30 |
| L-Arginine hydrochloride |
84 |
84 |
84 |
84 |
84 |
84 |
|
L-Cystine 2HCl |
63 |
63 |
63 |
63 |
63 |
63 |
|
L-Glutamine |
584 |
584 |
584 |
584 |
584 |
584 |
|
L-Histidine hydrochloride-H2O |
42 |
42 |
42 |
42 |
42 |
42 |
|
L-Isoleucine |
105 |
105 |
105 |
105 |
105 |
105 |
|
L-Leucine |
105 |
105 |
105 |
105 |
105 |
105 |
|
L-Lysine hydrochloride |
146 |
146 |
146 |
146 |
146 |
146 |
|
L-Methionine |
30 |
30 |
30 |
30 |
30 |
30 |
|
L-Phenylalanine |
66 |
66 |
66 |
66 |
66 |
66 |
|
L-Serine |
42 |
42 |
42 |
42 |
42 |
42 |
|
L-Threonine |
95 |
95 |
95 |
95 |
95 |
95 |
|
L-Tryptophan |
16 |
16 |
16 |
16 |
16 |
16 |
|
L-Tyrosine disodium salt dihydrate |
104 |
104 |
104 |
104 |
104 |
104 |
|
L-Valine |
94 |
94 |
94 |
94 |
94 |
94 |
Vitamins
|
Choline chloride |
4 |
4 |
4 |
4 |
4 |
4 |
|
D-Calcium pantothenate |
4 |
4 |
4 |
4 |
4 |
4 |
|
Folic Acid |
4 |
4 |
4 |
4 |
4 |
4 |
|
Niacinamide |
4 |
4 |
4 |
4 |
4 |
4 |
|
Pyridoxine hydrochloride |
4 |
4 |
4 |
4 |
4 |
4 |
|
Riboflavin |
0.4 |
0.4 |
0.4 |
0.4 |
0.4 |
0.4 |
|
Thiamine hydrochloride |
4 |
4 |
4 |
4 |
4 |
4 |
|
i-Inositol |
7.2 |
7.2 |
7.2 |
7.2 |
7.2 |
7.2 |
Inorganic Salts
|
Calcium Chloride (CaCl2) (anhyd.) |
200 |
200 |
200 |
200 |
200 |
200 |
|
Ferric Nitrate (Fe(NO3)3"9H2O) |
0.1 |
0.1 |
0.1 |
0.1 |
0.1 |
0.1 |
|
Magnesium Sulfate (MgSO4) (anhyd.) |
97.67 |
97.67 |
97.67 |
97.67 |
97.67 |
97.67 |
|
Potassium Chloride (KCl) |
400 |
400 |
400 |
400 |
400 |
400 |
|
Sodium Chloride (NaCl) |
6400 |
6400 |
6400 |
6400 |
6400 |
6400 |
|
Sodium Phosphate monobasic (NaH2PO4-H2O) |
125 |
125 |
125 |
125 |
125 |
125 |
Other Components
D-Glucose (Dextrose)
4500
4500
4500
4500
4500
4500
Phenol Red
15.9
15.9
15.9
15.9
15.9
15.9
Sodium Pyruvate
110
110
0
0
110
110
Sodium Bicarbonate (NaHCO3)
3700
3700
3700
3700
3700
3700
HEPES
0
5958
0
0
0
0
